METAB – diversity panel
The METAB assay was designed to analyze a broad range of metabolites. This method targets around 200 metabolites including acetylcarnitines, amino acids and their derivatives, biogenic amines, carboxylic acids, cofactors, nucleosides/nucleobases, nucleotides, phenyl acids, sugar derivatives, sugar phosphates, sugars, vitamins, and other metabolites. In contrast to the metabolite-specific assays, this assay is based on only one MRM transition, the quantifier, and is quantitative for some and semi-quantitative for other metabolites. For more information, please reach out. We perform data analysis for you.
LIPID – Targeted/Untargeted lipidomics method
This assay provides a semi-quantitative analysis for several lipid classes, referring to one isotopically labeled internal standard per class. Metabolites included in this method: phospholipids (PC, PE, PS, PG), sphingolipids (SM, Cer), glycerolipids (DG, TG), sterol-esters (CE). The general panel was expanded to also include glycosphingolipids and free cholesterol. More lipid classes can be added to the method upon request. For more information, please reach out. We perform data analysis for you.
AMINO – Targeted method for amino acid and derivates
This is a focused analysis of amino groups containing metabolites that are performed by HILIC-based separation coupled to a high-resolution mass spectrometer. Absolute quantitation is ensured by external calibration and isotopically labeled internal standardization. The metabolites included in this method are amino acids and their derivatives, polyamines, and acylcarnitines. While most amino acids are also included in the METAB02 method, AMINO01 is more sensitive and selective. For more information, please reach out. We perform data analysis for you.
CYCLE – Targeted method for TCA and Urea Cycle
This method is designed to monitor metabolites involved in the tricarboxylic acid (TCA) and Urea Cycle. Absolute quantification of TCA and urea cycle metabolites is based on external calibration and isotopically labeled internal standardization; ornithine, citrulline and aspartate are quantified semi-quantitatively (*). Metabolites included in this method are from the TCA cycle: pyruvate, citrate, cis-aconitate, isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, and itaconate; from the urea cycle: arginine, ornithine*, citrulline*, aspartate*, and additionally S-Adenosyl-Methionine. For more information, please reach out. We perform data analysis for you.
FACID – Targeted method for fatty acids
Focused analysis of fatty acids performed by derivatization of free fatty acids, separation via reversed phase liquid chromatography, and detection with a high mass accuracy mass spectrometer. Absolute quantification is based on external calibration and isotopically labeled internal standardization (N=35). For some fatty acids, the analysis is only semi-quantitative (N=3). For more information, please reach out. We perform data analysis for you.
BILAC – Targeted method for bile acids
Focused analysis of 32 bile acid species performed using optimized reversed phase HPLC and triple quadrupole mass spectrometry. Absolute quantification is based on external calibration and isotopically labeled internal standardization. Mouse specific primary bile acids α- and ω-muricholic acid cannot always be separated and are quantified as a sum while secondary conjugated muricholic bile acids are quantified individually. The metabolite panel for this method is still being expanded to include bile acids of special interest to customers like tetra-hydroxylated bile acids. For more information, please reach out. We perform data analysis for you.
CARN – Targeted method for carnitines
Focused analysis of acylcarnitines performed by derivatization to butyl esters, and after reversed phase liquid chromatography coupled to triple quadrupole mass spectrometer. Absolute quantification is based on deuterated internal standards and external calibration curves. For more information, please reach out. We perform data analysis for you.
Customized assays
Customized assays include compound analysis for synthetic organic chemists, targeted assays for specific metabolite classes, and tracing analysis based on isotopically labeled metabolites. For more information, please reach out. We perform data analysis for you.
Tracing experiments
Using high resolution mass spectrometry, we can perform the analysis of the protonated or deprotonated metabolite theoretical masses for molecular ions and isomers according to the incorporation of heavy carbon or nitrogen atoms. For more information, please reach out. We perform data analysis for you.
